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The cost for outer PCR is about $25 $30.50 a sample. You can follow this design to produce amplicons compatible with our indexing oligos.
Please note, due to increased sequencing cost and library prep reagents the cost of this prep has increased.
Metagenomics assay: This service starts with normalized (i.e. equal concentration and volume) gDNA and includes amplification using one of the GSAF provided primer sets chosen by the customer as shown on this web page. The service is calibrated to deliver at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 90% of the samples submitted, assuming there is reasonable amount of DNA to amplify. To minimize jackpot effects, PCR is performed in triplicate for each sample and re-pooled.
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| Number of Samples | Internal / UT Network | External Academic |
| 96 or more | $36$42.70 | $45$53.4590 |
| 1-95 samples | Add $369 / project | Add $464 / project |
Outer PCR only is about
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$30.50 a sample for internal, $38.50 for external
IMPORTANT CAUTION: The V4/V5 region assay we use is KNOWN to amplify Eukaryotic 18S rRNA genes as well as bacterial 16S V4/V5 so choose your primer sets carefully.
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