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| Code Block |
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Nextera® DNA Sample Preparation Kit (Illumina) 1,2 Nextera® transposase sequences (FC-121-1031, FC-121-1030) 5’ TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG (a) Read 1 --> 5’ GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG (d) Read 2 --> Nextera® Index Kit - PCR primers (FC-121-1012, FC-121-1011) 5’ AATGATACGGCGACCACCGAGATCTACAC[i5]TCGTCGGCAGCGTC (c) i5 Index read --> 5’ CAAGCAGAAGACGGCATACGAGAT[i7]GTCTCGTGGGCTCGG <-- i7 Index read (b) Nextera® codes for entry on sample sheet: Some oligonucleotide sequences © 2007-2012 Illumina, Inc. All rights reserved. |
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<-- i7 Index read (b)
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Nextera codes for entry on sample sheet:
i5 bases in adapter | Nextera DNA i5 index name |
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Nextera XT i5 index name |
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Nextera Enrichment i5 index name |
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i5 bases for entry on sample sheet |
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TAGATCGC |
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N501 |
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S501 |
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E501 |
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TAGATCGC |
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CTCTCTAT |
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N502 |
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S502 |
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E502 |
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CTCTCTAT |
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TATCCTCT |
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N503 |
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S503 |
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E503 |
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TATCCTCT |
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AGAGTAGA |
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N504 |
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S504 |
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E504 |
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AGAGTAGA |
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GTAAGGAG |
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N505 |
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S505 |
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E505 |
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GTAAGGAG |
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ACTGCATA |
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N506 |
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S506 |
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E506 |
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ACTGCATA |
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AAGGAGTA |
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N507 |
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S507 |
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E507 |
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AAGGAGTA |
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CTAAGCCT |
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N508 |
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S508 |
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E508 |
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CTAAGCCT |
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i7 bases in adapter |
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Nextera DNA i7 index name |
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Nextera XT i7 index name |
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Nextera Enrichment i7 index name |
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i7 bases for entry on sample sheet |
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TCGCCTTA |
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N701 |
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N701 |
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N701 |
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TAAGGCGA |
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CTAGTACG |
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N702 |
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N702 |
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N702 |
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CGTACTAG |
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TTCTGCCT |
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N703 |
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N703 |
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N703 |
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AGGCAGAA |
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GCTCAGGA |
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N704 |
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N704 |
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N704 |
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TCCTGAGC |
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AGGAGTCC |
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N705 |
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N705 |
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N705 |
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GGACTCCT |
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CATGCCTA |
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N706 |
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N706 |
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N706 |
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TAGGCATG |
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GTAGAGAG |
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N707 |
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N707 |
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N707 |
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CTCTCTAC |
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CCTCTCTG |
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N708 |
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N708 |
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N708 |
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CAGAGAGG | ||||
AGCGTAGC | N709 | N709 | N709 | GCTACGCT |
CAGCCTCG | N710 | N710 | N710 | CGAGGCTG |
TGCCTCTT | N711 | N711 | N711 | AAGAGGCA |
TCCTCTAC | N712 | N712 | N712 | GTAGAGGA |
Barcodes (also known as Indexes)
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