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Illumina HiSeq

Some simple commands to use to search for adaptor-dimers:
In Illumina data:

Code Block

grep -c GATCGGAAGA <filename.fastq>

This works for a standard Illumina genomic DNA library made with TruSeq V3 Y adaptors, and since the Y adaptors are RC's on each end, works equally well for read 1 and read 2.