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i5 bases in adapter | Nextera DNA i5 index name | Nextera XT i5 index name | Nextera Enrichment i5 index name | HiSeq 2500 and MiSeq i5 bases for entry on sample sheet | NextSeq and HiSeq 4000 i5 bases for entry on sample sheet |
|---|---|---|---|---|---|
TAGATCGC | N501 | S501 | E501 | TAGATCGC | GCGATCTA |
CTCTCTAT | N502 | S502 | E502 | CTCTCTAT | ATAGAGAG |
TATCCTCT | N503 | S503 | E503 | TATCCTCT | AGAGGATA |
AGAGTAGA | N504 | S504 | E504 | AGAGTAGA | TCTACTCT |
GTAAGGAG | N505 | S505 | E505 | GTAAGGAG | CTCCTTAC |
ACTGCATA | N506 | S506 | E506 | ACTGCATA | TATGCAGT |
AAGGAGTA | N507 | S507 | E507 | AAGGAGTA | TACTCCTT |
CTAAGCCT | N508 | S508 | E508 | CTAAGCCT | AGGCTTAG |
i7 bases in adapter | Nextera DNA i7 index name | Nextera XT i7 index name | Nextera Enrichment i7 index name | i7 bases for entry on sample sheet (HiSeq, MiSeq, or NextSeq) | |
TCGCCTTA | N701 | N701 | N701 | TAAGGCGA | |
CTAGTACG | N702 | N702 | N702 | CGTACTAG | |
TTCTGCCT | N703 | N703 | N703 | AGGCAGAA | |
GCTCAGGA | N704 | N704 | N704 | TCCTGAGC | |
AGGAGTCC | N705 | N705 | N705 | GGACTCCT | |
CATGCCTA | N706 | N706 | N706 | TAGGCATG | |
GTAGAGAG | N707 | N707 | N707 | CTCTCTAC | |
CCTCTCTG | N708 | N708 | N708 | CAGAGAGG | |
AGCGTAGC | N709 | N709 | N709 | GCTACGCT | |
CAGCCTCG | N710 | N710 | N710 | CGAGGCTG | |
TGCCTCTT | N711 | N711 | N711 | AAGAGGCA | |
TCCTCTAC | N712 | N712 | N712 | GTAGAGGA | |
Barcodes (also known as Indexes)
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