One quick thing to check- under the Edit–> Before using this protocol you will need to created a few hotkeys. If you have not already done this, go to Liath's Protocol - Creating New Hotkeys
Also, you will need to have your units set to mm. To check go to Edit --> Preferences --> Units & Rulers dialogue, and set units to mm.
Open an image in photoshop - make sure colors in the tool bar are set to white and black, with black white on bottom. verify number in specifytop.
Open Specify and run a query to check to be sure that the number and the label information match up with the data in Specify.
FYI – the photoshop scripts Photoshop scripts that you will need for this process are kept in the DocLib on the network drive. Z:\DocLib\code_and_scripts\PhotoshopScripting\NSF_Scripts\Production\Single monitor versions
1. Set Image Levels
Click Ctrl+L and the levels window will open. Shift the left arrow to the right in order to make the background black (be careful not to obscure any dark details on your specimen – this may require zooming in on the specimen first, before setting levels). Next shift the right arrow to the left in order to correct for white. Leave the central arrow in the center.
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