...
Code Block | ||||
---|---|---|---|---|
| ||||
#Looking up nlaIII, we see it's cut site: # CATG' # 'GTAC #check if we see this cut site in the forward reads grep CATG Lib01_R1.fastq | wc -l #compare with total read count expr $(cat Lib01_R1.fastq | wc -l) / 4 |
Next run the program FastQC on the reads
Code Block | ||||
---|---|---|---|---|
| ||||
module load fastqc
mkdir raw_Fastqc_Restults
fastqc -o raw_Fastqc_Restults/ -f fastq Lib01_R*.fastq
#scp the resulting .html files to your personal computer to see
|