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  • Prepare target(DB) sequence.
    Code Block
    $ bowtie-build -C <FASTA file> <DB name>
  • Run bowtie. If you use fastq file (read sequences with quality scores),
    Code Block
    $ bowtie \-a \-C \-q \-t \--suppress 6 <DB name> <Query fastq filename> <output filename>
    If you want to ignore quality file, and use 'fasta' format reads,
    Code Block
    $ bowtie \-a \-C \-f \-t \--suppress 6 <DB name> <Query csfasta filename> <output filename>

Normal base reads

  • Prepare target(DB) sequence. $ bowtie-build <FASTA file> <DB name>
  • Run bowtie. If you use fastq file (read sequences with quality scores), $ bowtie -a -q -t --suppress 6 <DB name> <Query fastq filename> <output filename>
    If you want to ignore quality file, and use 'fasta' format reads,$ bowtie -a -f -t --suppress 6 <DB name> <Query csfasta filename> <output filename>

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