...
Overview
The allseqs_R1.fastq file has 2,434,300 sequences and required ~2-3 minutes to align to the human genome (depending on which aligner we chose) if we cranked up the amount of threading used. But, this is a relatively small file by NextGen standards, representing 0.06x coverage of the human genome. Consider a file 20x larger than this one, which will now take 40-60 minutes to align and represents just 1.2x coverage of the genome, and consider that we need 30-60 coverage to reliably call variants. How long are we willing to wait for alignments to process? 16h? 24h? We need to be able to distribute the work across multiple nodes, not just multiple processors.
...