Post-translational modification (PTM) identification
Identify Identify and analyze PTMs in large-scale, 12 hour minimum ($564 internal, $720 external) per project.
1. List of modified and unmodified peptides with mass-shifts
Identify modified peptides along with the unmodified counterparts.
- Deliverables:
- reports generated by MODa (mass-shifts and peptide-spectral matches)
- Tools Used:
- MODa: (Na S et. al. ) used to identify mass-shifts on given peptides
2. Mapping mass-shifts to post-translational modifications and substitutions
Mapping the mass-shifts identified in step 1. to actual post-translational modifications and/or substitutions
- Deliverables:
- Mass-shift to PTM mapping
- Tools Used:
- ABRF delta mass: (Nesvhizskii et. al. ) used to identify PTMs mapping to mass-shifts identified above (i.e., 80 Dalton mass-shift on Serine indicates phosphorylation)
- ABRF delta mass: (Nesvhizskii et. al. ) used to identify PTMs mapping to mass-shifts identified above (i.e., 80 Dalton mass-shift on Serine indicates phosphorylation)
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