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For more usage information, check the documentation here
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<fastx_command> -h will display the help screen for that command. |
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Eg: fastx_trimmer -h |
Note: When using fastx tools with Illumina HiSeq data from Casava 1.8, make sure to add -Q33 option. Fastx tools assumes quality scores with ASCII offset 64, but the data is Sanger encoded(offset 33), so this needs to be specified explicitly by adding the -Q33 flag.