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titleAnswer

J is the best base quality score character (Q=41)

It represents a probability of error of < 1/10^4 10^4 or 1/10,000

About compressed files

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Code Block
languagebash
titlegzip, gunzip exercise
# if the $CORENGS environment variable is not defined
export CORENGS=/work/projects/BioITeam/projects/courses/Core_NGS_Tools

# make sure you're in your $SCRATCH/core_ngs/fastq_prep directory
cd $SCRATCH/core_ngs/fastq_prep

# Copy over a small, uncompressed fastq file
cp $CORENGS/misc/small.fq .

# check the size, then compress it in-place
ls -lh small*
gzip small.fq

# check the compressed file size
ls -lh small*

# uncompress it again
gunzip small.fq.gz
ls -lh small*

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Expand
titleSetup (if needed)


Code Block
languagebash
# Setup (if needed)
export CORENGS=/work/projects/BioITeam/projects/courses/Core_NGS_Tools
mkdir -p $SCRATCH/core_ngs/fastq_prep
cd $SCRATCH/core_ngs/fastq_prep
cp $CORENGS/misc/small.fq .


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