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J is the best base quality score character (Q=41) It represents a probability of error of < 1/10^4 10^4 or 1/10,000 |
About compressed files
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# if the $CORENGS environment variable is not defined
export CORENGS=/work/projects/BioITeam/projects/courses/Core_NGS_Tools
# make sure you're in your $SCRATCH/core_ngs/fastq_prep directory
cd $SCRATCH/core_ngs/fastq_prep
# Copy over a small, uncompressed fastq file
cp $CORENGS/misc/small.fq .
# check the size, then compress it in-place
ls -lh small*
gzip small.fq
# check the compressed file size
ls -lh small*
# uncompress it again
gunzip small.fq.gz
ls -lh small* |
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