Page Comparison

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1. Navigate to http://www.ncbi.nlm.nih.gov/nuccore/NC_012582
   • click on the Send to down arrow (top right of page)
     • select Complete Record
     • select File as Destination, and Format FASTA
     • click Create File
       
   • in the Opening File dialog, select Save File then OK
     • Save the file on your local computer as NC_012582.fasta
2. Back on the main http://www.ncbi.nlm.nih.gov/nuccore/NC_012582 page
   • click on the Send to down arrow (top right of page)
     • select Complete Record
     • select File as Destination, and Format GFF3
     • click Create File
       
   • in the Opening File dialog, select Save File then OK
     • Save the file on your local computer as NC_012582.gff3
3. Repeat steps 1 and 2 for the 2nd chromosome
   
   • NCBI URL is http://www.ncbi.nlm.nih.gov/nuccore/NC_012583
   • use NC_012583 as the filename prefix for the files you save
   • you should now have 4 files:
     • NC_012582.fasta, NC_012582.gff3
     • NC_012583.fasta, NC_012583.gff3
       
4. Transfer the files from your local computer to TACC
   • On a Mac or Windows with a WSL shell, use scp
   • On Windows, use the pscp.exe PuTTy tool
   • See Copying files between TACC and your laptop
5. Combine the 2 FASTQ files into one using cat
   
   • cat NC_012582.fasta  NC_012583.fasta > vibCho.fasta
6. Combine the 2 GFF3 files into one using cat
   
   • cat NC_012582  NC_012583 > vibCho.gbk

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