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The default calibration frame settings are still being tested, but we recommend: 

NOTE: Take the dark frames only at the beginning of the night!!

Typeexposure time (s)numberNumber of Fowler Reads
Dark301016
Flat off301016

Flat

301016
ThAr303

16

Then in a second set, with a different fowler number:
ThAr10 1531

UNe

1.63 1531