...
DNA ~400 bp insert size. Price includes final QC by BioAnalyzer and qPCR. See this web page for sample input guidelines.
RNA Size range typically ~200 nt; "dUTP" directional protocol. Price includes QC by BioAnalyzer on final lib only and qPCR. Please request a BA if you need to check the quality of the RNA before we proceed to library prep. See this web page for sample input guidelines.
Prices listed are per sample. For projects with 8 or less samples, a flat fee per project is charged as shown.
...
Contact the GSAF and we will be happy to plan out a project for your research goals.
| Anchor | ||||
|---|---|---|---|---|
|
The cost for outer PCR is about $30.50 a sample. You can follow this design to produce amplicons compatible with our indexing oligos.
...
Metagenomics assay: This service starts with normalized (i.e. equal concentration and volume) gDNA and includes amplification using one of the GSAF provided primer sets chosen by the customer as shown on this web page. The service is calibrated to deliver at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 90% of the samples submitted, assuming there is reasonable amount of DNA to amplify. To minimize jackpot effects, PCR is performed in triplicate for each sample and re-pooled.
The library preparation is a two-step process, a gene-specific PCR then a second PCR to add the dual indexes. The PCR targets the bacterial 16S V4/V5 region, the 16S V4 region, or fungal ITS region, as specified by the client. Gel-based QC is performed on a sampling of 10% of the samples post amplification. Genomic DNA is expected as input; the GSAF does not provide DNA extraction services at this time. See this web page for sample input guidelines and note that DNA concentrations should be normalized before submission. The GSAF can normalize your sample concentrations for an additional charge.
...