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If you are submitting this... | Then submit in this... | For this platform... | GSAF requests this amount... | In this volume... |
|---|---|---|---|---|
| Finished NGS sequencing libraries | All Final libraries must be in 1.5 ml tubes | Illumina NovaSeq, NextSeq, MiSeq, or iSeq | >2nM | ≥20ul |
| DNA or RNA for QC | 1.5 ml tubes | Bioanalyzer-DNA | >50pg/ul | 3-5ul |
| Bioanalyzer-RNA Nano | 25-500ng/ul (Total RNA) 25-250ng/ul (mRNA) | |||
| Bioanalyzer-RNA Pico | .05-5ng/ul (Total RNA) .25-5ng/ul (mRNA) | |||
| Bioanalyzer-small RNA | 1-100ng/ul (Total RNA) 1-20ng/ul (enriched small RNA) | |||
| DNA - genomic DNA (NOTE: this assumes PURE POPULATIONS - i.e. 100% bacterial or fungal - you may need to assay samples with mixed populations prior to submission) | 96-well plates*, or 1.5mL tubes if you are submitting ≤12 samples | Metagenomics (bacterial or fungal) | 10ng/ul FOR ALL SAMPLES SUBMITTED (i.e. customer MUST normalize input samples) | ≥25ul |
| DNA - genomic DNA, high molecular weight, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* | DNA library prep, Covaris fragmentation | 1-80ng/ul | ≥25ul |
| DNA fragments (including IP-derived DNA) or DNA amplicons (PCR products), for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* | DNA library prep | 1-80ng/ul | ≥25ul |
| RNA - total RNA, for library prep** | 1.5 ml tubes, or 96-well plate for 12 or more samples* | RNA-seq, PolyA purification | 10-80ng/ul | ≥25ul |
| RNA-seq, Ribosomal Depletion | 10-80ng/ul | ≥25ul | ||
| RNA-Seq, no removal or enrichment | 1-200ng/ul | ≥15ul | ||
| TagSeq | 25ng/ul (submitting RNA as low as 10ng/ul or as high as 100ng/ul is acceptable BUT ALL samples MUST be submitted at the same or very similar concentrations) Ideally you would submit at least 25ng/ul | ≥25ul | ||
| RNA-mRNA, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* | RNA-Seq | .2-20ng/ul | ≥15ul |
| RNA-miRNA or other RNA sub-fractions, for library prep | 1.5 ml tubes, or 96-well plate for 12 or more samples* | Small RNA library prep | 20-200ng/ul(Total RNA) 2-20ng/ul (purified small RNA) | ≥15ul |
* Samples should be plated starting at A1 then plate the samples vertically, A1 to H1, then A2 to H2 and so on.....Do not skip wells, you will be charged for wells that are skipped and we will not process samples that are plated A1 to A12. ** RNA must be free of DNA | ||||
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Always place tubes or plates in a secondary container, never ship samples loose in a box of dry ice!
Shipping
Shipping information is available here
Sample purity
We encourage you to evaluate purity (e.g. OD260/280 or OD230/260/280) as a measure of your nucleic acid extraction process. We do not provide guidelines for these measures because most of our NGS procedures begin with steps that are highly tolerant of contaminants, followed by purifications. If your samples fail our in-process QC while internal controls do not, we will contact you to troubleshoot further.