The GSAF is a full service sequencing core that provides both Sanger and Next Generation Sequencing services, in addition to a library prep and quality check services, open access to equipment and training. The GSAF works directly with the bioinformatics core who can provide assist in any of your analysis needs to data storage solutions.
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- Illumina NovaSeq 6000 (one)
- Illumina MiSeq sequencers (three)
- Illumina NextSeq 500 sequencer (two)
- Covaris S220 and E220 Adaptive Focused Acoustic shearing devices
- Agilent BioAnalyzer 2100Agilent TapeStation
- Invitrogen Qubit fluorimeter and Thermoscientific Fluoroskan
- Tecan Evo and Hamilton Nimbus
- 10x Chromium systemIon Torrent S5
Lab protocols we are experienced with:
- Creation of fragment DNA sequencing libraries for all Illumina next-gen sequencersCreation of mate-pair sequencing libraries between 1.5kb and 4kb for Illumina next-gen sequencers
- Creation of RNA-seq libraries from total RNA, small RNA, and immuno-precipitated RNA for Illumina next-gen sequencers
- Creation of amplicon libraries (particularly 16s and ITS regions) for the Illumina next-gen sequencers
- Human exome and custom capture with the Agilent SureSelect, Illumina TruSeq, and Nimblegen SeqCap EZ kits
- Sample and library QC using the Agilent BioAnalyzer, Picogreen and Ribogreen fluorimetry, qPCR, and spectrophotometry
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| Gabriella Huerta
Program Manager
(512) 475-9725 |
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| Gabriella manages the Illumina sequencing operations and contributes to all aspects of the genomic sequencing enterprise. Every library barcode, concentration and quality control metric is carefully scrutinized by Gabriella, as she performs the complex task of setting up the sequencing runs to assure the highest quality results. With prior experience running NGS operations at Perlegen Sciences and Pfizer, Gabriella has the depth of experience to get the jobs done right. |
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| Holly Stevenson
Research Engineering Scientist Associate IV |
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| Holly is a PhD scientist with prior experience applying and tailoring molecular protocols to cancer genetics research during her time as a graduate student and postdoc. Her interests expanded to include genomics and she subsequently served as a Biologist in a core facility for 5 years at the National Cancer Institute, preparing NGS libraries and implementing microarrays for both research and clinical studies. Since joining GSAF in 2017, she has assisted in testing and expanding new services, including TagSeq and 10X Genomics single-cell technologies. She currently operates the Illumina Novaseq sequencer, manages and performs runs for single-cell studies, and aids customers in project planning and troubleshooting. |
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| Aditi Karmakar
Research Engineering Scientist Associate III |
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| Aditi has a master's degree in Genetic Engineering from India and has over 11 years of experience in molecular biology techniques. She has been under UT community for 5 years, and previously she has extensive experience in understanding and handling DNA and RNA for various Research purposes both in and outside UT. She was looking at how different environmental factors like; diet and alcohol can affect CNS inflammatory disorders like Multiple sclerosis in Melamed lab in UT and that experience gave her an immense knowledge on various molecular mechanisms as well as the variety of techniques that are used to study them. She has also worked as one of the technicians in Sanger core and processed samples for sequencing and Fragment analysis. She is currently involved in a variety of DNA/RNA library preparations and operating our Illumina Miseq sequencers. |
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| María Jose Gomez
Research Engineering Scientist Associate II
(512) 475-9725 |
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| María attended the Universidad de los Andes in Colombia where she completed her studies on Biology with a minor in Bioinformatics. Before joining the GSAF her work included a variety of molecular techniques on systems such as rodents, frogs, bacteria, viruses and plants. As part of the GSAF her focus is on NGS library preparation, including DNA libraries, RNA-Seq, TagSeq and quality control of said libraries.
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| Zachary Carver
Research Engineering Scientist Associate II |
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