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An example of a custom genome database set up with SequenceServer can be found here.

Enter in the following sequence:

CGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCT
GATAAGCGCAGCGCATCAGGCAATTTGGCGTTGCCGTCAGTCTCAGTTAATCAGGTTACAACGATTAACC
CTGCAGCAGAGACAGAACCTGCTGCGGTACCTGGTTAGCTTTTGCCAACACGGAGTTACCGGCCTGCTGG
ATGATCTGCGCTTTCGACATATTGGACACTTCGGTCGCATAGTCGGCGTCCTGAATACGGGACTGCGCTT

Select blastn as your blast method, check NC_000913.2.fasta as your nucleotide database and finally click BLAST!

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