Ultramicrotome and Serial Sectioning

Know Thyself

When and/or under what conditions are your hands most stable?
- Make sure you get enough sleep.
- Make sure you are hydrated and fed enough.
Practice using forceps, eyelash, and ultramicrotome until you feel like they are extensions of your fingers.
- Manipulating grids with forceps under different light conditions. You need to be able to pick one up, flip it over, hold it still, insert it into a slot on wax surface, etc. Also, practice using a grid on forceps to move and orient ultrathin sections at will.
- Using an eyelash under binoculars at different magnifications to manipulate ultrathin sections on water surface. You need to be able to tap on to a section without breaking it. Can you use it to move around a section on water surface, or stop one from moving?
- Ultramicrotome – know which knob to turn to get your block face aligned to the knife without thinking. Get to know the illumination system by trying different settings. Make sure you know how to adjust binoculars to your eyesight. Get to know the field of view at different magnification – at the highest mag, you can see more details, but the field is too small to work with an eyelash effectively and efficiently. Figure out the best ergonomics for you – you need to be able to hold an eyelash steady without straining your shoulders and back.

References

Sitte H (1982) Ultramicrotomy - Common problems and mistakes. PDF

Peachey LD (1958) Thin sections. I. A study of section thickness and physical distortion produced during microtomy. J Biophys Biochem Cytol 4:233-242. PDF