Lab Equipment Maintenance Schedule [Olga]

Lab Equipment Maintenance Schedule [Olga].docx

Equipment Maintenance Schedule

Weekly:

• Check/Clean gaskets in all -80°C Freezers Rm 1.132, 1.122
• Add Liquid Nitrogen to Cell Culture Tank to cover boxes. Alarm is not repairable. So, adding a little of LN2 weekly is a consistent and easy way to keep it in order.
• Test eyewashes in 1.124 ; 1.106 and 1.128 (cold room)
  1. Initial Log once a month only
• Fill tip boxes
  1. Really done as needed, usually done by those who is using tips the most
• Check biohazard and send request to EHS or autoclave Biohazard bags if needed
• Dishes
  1. Undergrad duty (but all senior technicians need to be familiar with that, if undergrad needs to be trained)
• Re-Fill containers for 1.5ml, 0.5ml tubes
  1. This is done as needed
• Check water baths
• Check water carboys and fill as needed
  1. Oocyte group will help with this

Monthly:

• Log Eyewash Test
• Flush filtration system in 1.106
• Autoclave testing

Corina Hernandez will drop off the testing vials and form. There is a change how these tests are conducted:

Note: Notify Corina (chernandez@austin.utexas.edu  or call her 512-475-9722) as soon as you remove the test pack from the autoclave, the test pack needs to be placed in the  incubator as soon as possible after going through an autoclave cycle.

The test will no longer be valid if greater than 72 hours. You will need to repeat any tests that are not valid.

Every few months:

• Vacuum Filters on -80°C Freezers using wet-dry vac in autoclave room

Every 6 months

• Service pre-filtration system filtering tanks

Yearly:

• Service to e-Pure water filters (as needed, once system not holding 18mO
• Balance calibration
• PM on centrifuges
• Autoclave water carboys. Remove spigots and take them apart, rinse well, dry, place in beaker cover with foil and autoclave. Cover spigot opening of carboy with foil and autoclave.
• Change Silicone beads in all desiccators
• Defrost Freezers
• Complete Lab Self-Evaluation Form, EHS sends reminder to all lab managers and PIs.