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If all went well, you should now see a mqc_report.html file that looks like this: http://web.corral.tacc.utexas.edu/iyer/byteclub/multiqc/03_bowtie.mqc_report.html, with new sections for Picard and Samtools reports.

Fix the Picard MarkDuplicates sample name

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The resulting report should look like this: ftp://gapdh.icmb.utexas.edu/misc/multiqc/04.mqc_report.fixed_align_info.html, with a cleaned up General Statistics table.

Controlling report section order

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Code Block
titlemultiqc_config.yaml
# Titles to use for the report.
title: "ATAC-Seq QC Reports"
subtitle: null
intro_text: "MultiQC reports for Igor's ATAC-Seq proof-of-concept project."
report_header_info:
    - Sequenced by: 'GSAF'
    - Job: 'JA17277'
    - Run: 'SA17121'
    - Setup: '2x150'

# Change the output filenames
output_fn_name: mqc_report.html
data_dir_name: mqc_report_data

# Ignore these files / directories / paths when searching for reports
fn_ignore_files:
    - '*.dupinfo.txt'

# Modules that should come at the top of the report
top_modules:
    - 'generalstats'
    - 'fastqc'
    - 'samtools'
    - 'picard'

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Expand
titleCatch up

To catch up, just use Anna's pre-made files:

Code Block
languagebash
mkdir -p $SCRATCH/byteclub/multiqc/02_bowtie
cd $SCRATCH/byteclub/multiqc/02_bowtie
ln -s -f /work/01063/abattenh/projects/byteclub/multiqc/fastqc
rsync -avrP /work/01063/abattenh/projects/byteclub/multiqc/bowtie2/ bowtie2/
cp /work/01063/abattenh/projects/byteclub/multiqc/conf/05_section_order.yaml multiqc_config.yaml
mkdir for_multiqc
cp /work/01063/abattenh/projects/byteclub/multiqc/for_multiqc/brain*.dupmetrics.txt for_multiqc/


After making this config file modification, you can now run multiqc again:

Code Block
languagebash
cd $SCRATCH/byteclub/multiqc/02_bowtie; rm -rf mqc_report*; multiqc .

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