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Get your data

Code Block
titleGet set up
cds
mkdir my_rnaseq_course #this is where you'll be doing all the course exercises
cd my_rnaseq_course
cp -r /corral-repl/utexas/BioITeam/rnaseq_course_2016/day_1_partA .  #for this specific exercise
cd day_1_partA/tacc_exercise

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  1.  First you need all the files. One is missing. Download it from here -> genes.1  and transfer it to your lonestar5 account (under day_1_partA/tacc_exercise)

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    titleHint

    Use the scp command from your computer (or winscp if its a windows machine)

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    titleAnswer
    Code Block
    #FOR MAC: 
    #Open terminal
    #Go to where genes.1 got downloaded (most likely Downloads)
    cd ~/Downloads
    scp genes.1 <username>@ls5.tacc.utexas.edu:/scratch/*/<username>/my_rnaseq_course/day_1_partA/tacc_exercise
    
    
    #FOR WINDOWS:
    Use winscp (free to download) to drag and drop the files from your computer to the server (in the right directory).
  2. Make a commands file with the unix commands to print the first 10 lines of each file into an output file.

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    titleHint

    Open an editor to create a file called commands

    Use head command to print the first 10 lines.

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    titleAnswer
    Code Block
    nano commands
    
    head genes.1 > head.1
    head genes.2 > head.2
    head genes.3 > head.3
    head genes.4 > head.4 
     
    #ctrl+x to exit (then Y to save)
  3. Use launcher_creator.py to create a launcher to submit this job (command file).

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    titleHint

    launcher_creator.py -h to see the options.

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    titleAnswer
    Code Block
    launcher_creator.py -n exercise -t 01:00:00 -j commands -q development -a UT-2015-05-18
  4. Submit the job.

    Warning

    Use our summer school reservation when submitting jobs to get higher priority:

    sbatch --reservation=CCBBintro_Day_1NGS

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    titleHint

    sbatch

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    titleAnswer
    Code Block
    sbatch exercise.slurm



  5. Check the queue.

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    titleAnswer
    Code Block
    showq -u <username>
  6. Check the output files and log files.

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    titleHint

    Look at <jobname>.o* file for output information

    Look at <jobname>.e* file for log/error information

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    titleAnswer
    Code Block
    less exercise.o* #q to exit
    less exercise.e* #q to exit

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