GO Enrichment
Clustering using WGCNA
Tag-Seq
Tag-Seq is targeted sequencing of just the 3' ends of mRNA.
- This is done at GSAF using a template switching protocol.
- It offers a reduction in library prep and sequencing cost.
- Consider it when looking to do differential expression analysis of known genes with more replicates, conditions, timepoints etc.
- Analysis involves some extra preprocessing. Other steps are similar to standard RNA-Seq analysis.