Sample Requirements

Sanger Sequencing

A.  Individual Samples (<24 samples)

• • Use a 1.5 mL microfuge tube
  • Provide at least 10 uL of sample (12-15 uL preferred)
  • Centrifuge your sample before dropping it off at DSF
  • Write your order number and sample NUMBER legibly on the cap             

B.  96 Well Plate (>24 samples)

• • Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
  • Provide at least 10 uL of sample (12-15 uL preferred)
  • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
  • Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
  • Write your order number legibly on the plate and foil
  • Put your samples in order vertically, starting with well A1
        

PCR Cleanup

 A.  Individual Samples (<24 samples)

• • Use a 1.5 mL microfuge tube
  • Provide at least 20 uL of unpurified PCR reaction
  • Centrifuge your sample before dropping it off at DSF
  • Write your order number and sample NUMBER legibly on the cap

• • Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
    
  • Primer should be provided at 10 uM concentration  
  • Write the order number AND primer name legibly on the cap.                                      

B.  96 Well Plate (>24 samples)

• • Use a 96-well PCR (i.e. conical well) plate (DSF uses Eppendorf twin-tec 951020600)
  • Provide at least 20 uL of unpurified PCR reaction
  • Seal your plate with adhesive foil (DSF uses VWR 60941-074)
  • Centrifuge your plate before dropping it off (you can use our Sorvall Legend X1)
  • Write your order number legibly on the plate and foil
  • Put your samples in order vertically, starting with well A1

• • Provide at least 3 uL of primer per sample in a 1.5 mL microfuge tube
    
  • Primer should be provided at 10 uM concentration  
  • Write the order number AND primer name legibly on the cap