Coating microscope slides with gelatin

under construction

based on http://www.ihcworld.com/_protocols/histology/gelatin_slides.htm

Coating:

Chrome Alum Gelatin Solution:

      Gelatin, type A, 220 or 275 Bloom -------- 1.5 g

      Chromium potassium sulfate -------------- 0.25 g

      Distilled water ------------------------------- 500 ml

Heat the water to 60 ºC and completely dissolve the gelatin with the aid of a magnetic stirrer. Stir in the chromium potassium sulfate (The solution should turn a pale blue). Add a few crystals of thymol as a preservative.

Procedure:

Dip racks of clean slides in the warm gelatin solution (40-50 ºC), drain the slides onto paper tissue, and then stand the slides (covered with foil to keep off dust) on end to air dry or 37 ºC incubator overnight. Store in dust-free container at room temperature. Throw out the gelatin mixture after use.

Mounting vibratome sections (20-50um):

Mount the sections on gelatin-coated slides and air dry overnight or 2-3 days.