3D Scene
I) Adding an object to the 3D scene | |
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1) Open the object list (Lists → Object List OR shift + CTL + o) | |
2) Inside the object list, right click on the object you want to add to the 3D scene → 3D → Add to scene | |
3) A separate 3D scene window containing a 3D reconstruction of your object should open (note – if a window does not appear, check behind your PyReconstruct window):
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II) Removing an object from the 3D scene | |
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1) Right click on object in object list → 3D → Remove from scene |
III) Editing 3D object | |
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1) Edit object 3D scene attributes by right clicking on object in object list → 3D → Edit 3D settings | |
2a) A 3D object settings window will open. Here you can change the 3D type and opacity (how transparent your object is). For most objects you will want 3D Type: Surface. However, for some object types you may want to select 'spheres' or 'contours' (see 2b, 2c) | |
2b) For vesicles, you will want to change your 3D Type to spheres | |
2c) In some cases, you may want to view your object's as its 2D contours stacked in 3D. | |
3) To change the color of your object in the 3D scene you need to change your object attributes: Right click → Edit attributes. --Note the object will need to be removed from the 3D scene and added back in if you want your 3D scene window to reflect the attribute changes |
IV) Navigating the 3D scene window (Scale Cube, Obj Transparency) | |
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1) Access the 3D scene help menu: Click on 3D scene window → press shift + ? key
Highlights
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V) 3D scene smooth settings | |
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1) 3D scene smoothing type/method used by PyReconstruct can be change by going to Series → 3D → Change smoothing type | |
a) Humphrey (less smooth) | |
b) Laplacian (most smooth) | |
c) None (blocky) |