Picard

Learn more about Picard's Mark Duplicates

If you want to run Picard's Mark Duplicates on Lonestar, do roughly this:

module load picard
# "picard" is not the name of the tool; picard is a collection of java .jar files that do things, so we have to figure out where they are and which ones they are, like this:
ls $TACC_PICARD_DIR
# Now to run Mark Duplicates, do something like:
java -Xmx2g -jar $TACC_PICARD_DIR/MarkDuplicates.jar INPUT=all.bowtie.sorted.bam OUTPUT=all.bowtie.sorted.dedup.bam METRICS_FILE=all.bowtie.sorted.dedup.bam.metrics ASSUME_SORTED=TRUE

The output metrics file is required and worth screening before you go further.

Learn more about picard on Fourierseq

Picard is also installed on fourierseq at /usr/local/genome/bin and the jar files are in fourierseq user's paths.

General syntax for using picard jar files:

java -Xmx4g -jar /usr/local/genome/bin/jarfilename

Find picard document here